My Western blots are not running proper. Any ideas what could it be and how to get rid of that? | ResearchGate
![Ran a western blot (protein) with cell lysate and conditioned media in MES buffer. Trying to troubleshoot. What is this band at the bottom? : r/labrats Ran a western blot (protein) with cell lysate and conditioned media in MES buffer. Trying to troubleshoot. What is this band at the bottom? : r/labrats](https://preview.redd.it/ran-a-western-blot-protein-with-cell-lysate-and-conditioned-v0-jqplokf99pea1.jpg?auto=webp&s=4ad4455e1540f92cb415cb19129b0b70b41664db)
Ran a western blot (protein) with cell lysate and conditioned media in MES buffer. Trying to troubleshoot. What is this band at the bottom? : r/labrats
![Is yellow and blue dye separating during a western blot normal? Using MES gel buffer, LDS sample loading buffer (loaded conditioned DMEM media from transiently transfected cells) : r/labrats Is yellow and blue dye separating during a western blot normal? Using MES gel buffer, LDS sample loading buffer (loaded conditioned DMEM media from transiently transfected cells) : r/labrats](https://preview.redd.it/is-yellow-and-blue-dye-separating-during-a-western-blot-v0-kco0hoon2gja1.jpg?width=640&crop=smart&auto=webp&s=bb8f1d83a6f7a262572caca61580e8b1ba485418)